The In-vitro Antibiotic Sensitivity Test of Pasteurella multocida Isolated from Layer and Breeder Chickens

Document Type : Short Communication


1 Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

2 Central Laboratory for Evaluation of Veterinary Biologics, Cairo, Egypt

3 Poultry Diseases Department, Faculty of Veterinary Medicine, Cairo University, Egypt


This work was designed to characterize Pasteurella multocida (P. multocida) isolates from layers and breeders chickens flocks in Egypt with emphasis on in-vitro antibiotic sensitivity and resistant pattern. Liver, heart, spleen, and lungs were collected aseptically from diseased birds suffering from respiratory manifestations, septicemia, drop in egg production, and mortalities in the period from 2016-2017. Samples were cultured on modified Das media for isolation of bacteria. Pure colonies of P. multocida isolates were identified according to microscopic morphology and biochemical characters. The isolated P. multocida were subjected to in-vitro antibiotic sensitivity test. Out of 330 examined chickens, 36 isolates were positive for P. multocida. Cultural study revealed small glistering, grayish, mucoid, and dew drop P. multocida colonies. Microscopically, P. multocida isolates were Gram negative coccobacilli. All the isolates were positive for catalase, oxidase, indol production, nitrate reduction, and H2S production tests, while negative for methyl red, Voge’s proskaur, urease activity, and gelatin liquefaction tests. Moreover, they fermented glucose, fructose, mannose, mannitol, sucrose, sorbitol, and xylose without gas production but not ferment arabinose, inositol, lactose, maltose, salicin, dulcitol and raffinose. Isolated P. multocida strains were sensitive to ofloxacin, tetracycline, trimethoprim/sulphamethoxazole, penicillin, chloramphenicol, norfloxacin, azithromycin, and erythromycin, while resistant to ampicillin and clindamycin. Intermediate sensitivity was observed for cefoperazone, gentamycin, and streptomycin.