The in-vitro Antibiotic Sensitivity Test of Pasteurella multocida Isolated from Layer and Breeder Chickens

Abstract

The current study aimed to characterize Pasteurella multocida (P. multocida) isolates from layers and breeder chickens in Egypt regarding in-vitro antibiotic sensitivity and resistance pattern. In doing so, spleen, liver, lungs, and heart, were taken aseptically from chickens suffering from a drop in egg production, septicemia, respiratory manifestations, and mortalities between 2016 and 2017. To isolate bacteria, samples were grown on a modified Das medium. Moreover, microscopic appearance and biochemical characteristics were used to identify pure colonies of P. multocida isolates. In the next step, in-vitro antibiotic sensitivity testing was performed on the isolated P. multocida. The findings indicated that P. multocida was found in 36 isolates out of 330 investigated chicken flocks. Small glistering, mucoid, grayish, and dew drop P. multocida colonies were discovered during the culture analysis. Pasteurella multocida isolates were Gram-negative coccobacilli using the microscope. Catalase, indole generation, H2S production, nitrate reduction, and oxidase tests were all positive for the sample; however, methyl red, urease activity, Voge's proskaur, and gelatin liquefaction tests were all negative. They also fermented glucose, mannose, fructose, sucrose, mannitol, xylose, and sorbitol without producing gas but not lactose, arabinose, maltose, inositol, salicin, raffinose, or dulcitol. Isolated P. multocida strains were sensitive to tetracycline, erythromycin, trimethoprim/sulphamethoxazole, norfloxacin, ofloxacin, penicillin, chloramphenicol, and azithromycin, while resistant to ampicillin and clindamycin. Cefoperazone, gentamycin, and streptomycin all showed intermediate sensitivity.