_1731571409 20241114080329000 Rovedar Daryoushbabazadeh@gmail.com Rovedar Journal of World’s Poultry Science J. World's Poult. Sci. 2980-7999 09 29 2024 3 3 Herve Tchoffo https://orcid.org/0000-0001-8678-5563 Chongsi Margaret Mary Momo https://orcid.org/0000-0003-2863-4905 Pride Forsoh Ayemle https://orcid.org/0009-0009-5933-8739 Roussel Manfouo https://orcid.org/0009-0009-4789-9248 Camile Kondo Nyembo https://orcid.org/0000-0003-0099-2794 Mohamadou Adamou https://orcid.org/0000-0001-6840-5010 Byamungu Kasomo Dedieu https://orcid.org/0009-0002-6843-9872 Arius Baulland Nguedia Dongmo https://orcid.org/0000-0002-7219-572X Ferdinand Ngoula https://orcid.org/0000-0002-6998-1957 Introduction: Soursop leaves are rich in various molecules, including total phenols, terpenes, and steroids, which possess a range of pharmacological properties that can be utilized in animal production to enhance both growth and reproduction of animals. The present study aimed to evaluate the effect of incorporating soursop (Annona muricata; A. muricata) leaves powder into feed on the reproductive performance of Japanese quail. Materials and methods: A total of 80 Japanese quails (64 females and 16 males) aged two weeks were randomly divided into four experimental groups, labeled T0, T1, T2, and T3, and received feed additives with 0 mg/kg body weight (bw), 250 mg/kg bw, 500 mg/kg bw, and 750 mg/kg bw of powdered soursop leaves, respectively. Additionally, water was provided ad libitum, and the quails' weights were measured every 7 days for a period of 75 days. At the end of the period, 12 female quails from each group were sacrificed after 24 hours fasting period. Blood was collected for hematological (Leukocyte, Erythrocyte, and Platelet indices) and serum biochemical (total serum cholesterol, total proteins, albumin, and globulin, Aspartate aminotransferase, Alanine aminotransferase, Urea, and Creatinine) analysis. The males were also sacrificed to evaluate the spermatozoa characteristics (mobility, concentration, and viability). Results: No statistical significant changes in growth characteristics or hematological parameters were observed. However, biochemical parameters increased significantly with the inclusion of Soursop (A. muricate) in quail feed, including increased total cholesterol, total protein, and globulin levels, and decreased malondialdehyde levels. This effect was most significant at dosage of 500 mg/kg bw. Serum levels of urea, Alanine aminotransferase, and Aspartate aminotransferase were not significantly affected by A. muricata whatever the concentration considered. A significant increase in fast progressive spermatozoa, along with a decrease in immotile spermatozoa, was observed with A. muricate at dosage of 500 mg/kg bw compared to the control. Sperm viability also increased significantly, particularly in live at dosage of 500 mg/kg bw. Significant increase was observed in fertility parameters, including increased fertility rate, hatchability rate of fertile eggs, total hatchability rate, and chick weight, along with decreased embryonic mortality at 500 mg/kg bw treatment compared to the control. Conclusion: In conclusion, the findings indicated that incorporating A. muricata leaf powder at 500 mg/kg bw into quail feed positively influences reproductive cells and boosts fertility growth promoters. 09 29 2024 42 52 https://creativecommons.org/licenses/by/4.0 10.58803/jwps.v3i3.39 https://jwps.rovedar.com/index.php/JWPS/article/view/39 https://jwps.rovedar.com/index.php/JWPS/article/download/39/62 https://jwps.rovedar.com/index.php/JWPS/article/download/39/62 Ministry of livestock, fisheries, and animal industries (MINEPIA). Document de stratégie du sous-secteur de l’élevage, des pêches et des industries animals [Strategic document for the Livestock, Fisheries, and Animal Industries sub-sector]. 2011, p. 77-87. Available at: https://faolex.fao.org/docs/pdf/cmr146473.pdf FOOD and agriculture organization of the United Nations (FAO). The state of world’s biodiversity for food and agriculture in Cameroon. FAO commission on genetic resources for food and agriculture. Available at: https://openknowledge.fao.org/server/api/core/ bitstreams/50b79369-9249-4486-ac07-9098d07df60a/content 10.1093/jac/dkg313 10.58803/fahn.v1i1.8 Nejib M, Taroub B, Imen O, François R, Mokhtar H, and Ridha B. Effet de deux préparations d'huiles essentielles sur la croissance des bactéries in vitro et Les performances du poulet de chair [Effect of two essential oil preparations on the growth of bacteria in vitro and the performance of broiler chickens]. Proceeding of 8th Poultry Research Days, 2009 March 25-26; p. 1-5. Available at: https://www.cabi.org/Uploads/animal-science/worlds-poultry-science-association/WPSA-france-2009/19_fpd2009_mathlouthi.pdf 10.1155/2018/7682060 10.1590/1806-9061-2023-1784 10.3390/molecules27041201 10.1155/2022/8828358 10.3390/foods12071448 10.58511/jnpdd.v1i1.5317 Rachael A. Soursop (Graviola): Health benefits and uses. Healthline Media. 2023. Available at: https://www.healthline.com/nutrition/ soursop-benefits 10.1155/2019/7857504 Banso A, and Ngbede JE. Phytochemical screening and in vitro antifungal properties of Fagara zanthoxyloides. J Food Agric Environ. 2006; 4(3): 71-78. Available at: https://www.cabidigitallibrary.org/ doi/full/10.5555/20063226013 Ngbede J, Yakubu RA, and Nyam DA. Phytochemical screening for active compounds in Canarium schweinfurthii (Atile) leaves from Jos North, Plateau State, Nigeria. Res. J Biol Sci. 2008; 33(3): 1076-1078. Available at: https://docsdrive.com/pdfs/medwelljournals/rjbsci/2008/1076-1078.pdf 10.20884/1.jap.2017.19.1.593 Jourdain R. Poultry in tropical environment. International Couloumiers. 1980; 43-45. 10.1007/s11250-011-9926-x 10.1590/1806-9061-2019-1045 10.3923/ijps.2023.174.182 Tchoffo H, Chongsi MMM, Omer BN, Christelle YDS, Mohamadou A, Nathalie N, et al. Effects of Dacryodes edulis and Mangifera indica leafes powders on immune system and growth performances 10.3923/ajpsaj.2023.9.19 10.21203/rs.3.rs-2903831/v1 10.51791/njap.v44i3.744 10.21608/epsj.2022.278520 10.5114/amsad.2020.103028 10.1016/j.joen.2016.08.017 10.53346/wjapmr.2023.4.1.0054 10.1093/humupd/2.3.246 10.1093/tas/txac046 10.6000/1927-5129.2013.09.69 Nguyen TG, Ngo TD, Do VAK, Le Thi TH, Pham PL, and Vo Thi K. Moringa leaf powder as natural feed additive on productive performances and egg quality of ISA Brown laying hens. Livest Res Rural Dev. 2023; 35(2): 15. Available at: https://www.lrrd.org/lrrd35/2/3515ntgi.html 10.1088/1755-1315/1262/7/072052 10.5897/JMPR09.464